FastQCFastQC Report
Fri 3 Aug 2018
H3VNWAFXY_n02_adr3wtzt14s92.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VNWAFXY_n02_adr3wtzt14s92.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences531411
Sequences flagged as poor quality0
Sequence length76
%GC55

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG46350.8722062584327385No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGAGT602.6289308E-740.7658238
AAGAATA400.002414717835.009232
TCTCTAG652.125036E-532.2542766
TCCTAGG450.004290598431.1333547
GTATTGT450.00434064131.0596751
TCTGAGC1700.028.7758758
ATCTGAG3200.028.3904827
ATCTGTG2150.027.6286627
TCTAGGC906.244156E-627.1772178
ATCTAGT1056.8593E-726.6225787
CGATCTG29000.026.0258665
AACGTAT959.435818E-625.77006719
CTGAGTG700.00123724424.9586689
GAACGTA1001.4044101E-524.48156418
CAGGGAT1151.508628E-624.32127614
CGTATTC1202.1738815E-623.32103521
TCCTACA750.00184897723.2947582
CTATTGG901.9479953E-423.2947581
TATACAG901.9479953E-423.2947585
GATCTGG11550.022.6896976
Produced by FastQC (version 0.11.2)