FastQCFastQC Report
Fri 3 Aug 2018
H3VNWAFXY_n02_adr3wtzt14s91.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VNWAFXY_n02_adr3wtzt14s91.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences529460
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG28790.5437615683904355No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTAGAC252.402357E-455.938823
ATCTTAC1201.8189894E-1234.9657257
GATCTTA1650.033.9061556
ACTATCT652.1152264E-532.2760545
GGGTACC450.00431432331.0982616
ATCCGTA450.004328706331.077121
TTTCGGA2850.030.6682131
CGATCTG21700.029.326095
TATTAGA853.985504E-628.7920382
CTATCTT500.007240176727.972586
TAGCGTC500.007240176727.972585
CTAGACT500.007244182827.969414
TTCCGAT56900.026.8511262
CCGATCT55750.026.6524644
ATAGTGT808.709971E-526.2272648
GTACTAT1207.4669515E-826.2213211
TCCGATC58550.026.094433
GATCTGG9200.025.0841056
TAGACGG700.00123237624.9755175
CTTCCGA61800.024.9484421
Produced by FastQC (version 0.11.2)