FastQCFastQC Report
Fri 3 Aug 2018
H3VNWAFXY_n02_adr3wtzt14s90.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VNWAFXY_n02_adr3wtzt14s90.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences571872
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG54050.9451415701415701No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCATAC350.001263362539.9462057
ATTCCTA552.9813295E-431.83546345
TGAGTGG553.0108454E-431.78205510
TGCTACG553.0108454E-431.78205510
CTGAGTG553.012698E-431.7787239
TTCTAAT450.00432996231.07578516
GATCTTT4050.031.069276
CGATCTG27300.028.6793275
GAGTCTT755.5289176E-528.03876767
GATACTC500.00720925727.99756632
CTTTTTT3500.027.9652779
TAGACTT500.007253700427.9623436
TCTTTTT3550.027.57148
CGATCTT11450.027.4739195
CCGATCT64650.027.1946224
ATCTTTT3750.027.0302667
TCTAGTG658.010324E-426.8896888
TCCGATC66750.026.7056083
TTCCGAT66700.026.6732232
TTTCGGA2900.026.5160161
Produced by FastQC (version 0.11.2)