FastQCFastQC Report
Fri 3 Aug 2018
H3VNWAFXY_n02_adr3wtzt14s8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VNWAFXY_n02_adr3wtzt14s8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7339
Sequences flagged as poor quality0
Sequence length76
%GC57

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCATTGCCGATCGGAAGAGCACACGTCTCATTGCCGATCGGAAGAGCA1301.7713584957078623Illumina Multiplexing Index Sequencing Primer (95% over 22bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1191.6214743153018123No Hit
GCAATGAGACGTGTGCTCTTCCGATCGGCAATGAGACGTGTGCTCTTCCG911.2399509469955035No Hit
CTTCCGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN741.0083117590952446No Hit
GTGCTCTTCCGATCGGCAATGAGACGTGTGCTCTTCCGATCGGCAATGAG690.9401825861834037TruSeq Adapter, Index 1 (95% over 22bp)
GAAGAGCACACGTCTCATTGCCGATCGGAAGAGCACACGTCTCATTGCCG670.9129309170186675Illumina Multiplexing Index Sequencing Primer (95% over 22bp)
CTTCCGATCTAGGCGGGAGACGTGTGCTCTTCCGATCTAGGCGGGAGACG560.7630467366126176Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTTCCGATCGGCAATGAGACGTGTGCTCTTCCGATCGGCAATGAGACGTG410.5586592178770949TruSeq Adapter, Index 2 (95% over 22bp)
GATCGGAAGAGCACACGTCTCCCGCCTAGATCGGAAGAGCACACGTCTCC370.5041558795476223Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
GAAGAGCACACGTCTCCCGCCTAGATCGGAAGAGCACACGTCTCCCGCCT310.42240087205341326Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
GTGCTCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN300.40877503747104516No Hit
GATCGGAAGAGCACACGTCTCATTGCCGATCGGAAGAGCACACGTCTCAT290.395149202888677Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
GACGTGTGCTCTTCCGATCGGCAATGAGACGTGTGCTCTTCCGATCGGCA290.395149202888677TruSeq Adapter, Index 2 (95% over 22bp)
GTGCTCTTCCGATCTAGGCGGGAGACGTGTGCTCTTCCGATCTAGGCGGG280.3815233683063088Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCTCCCGCCTAGATCGGAAGAGCACACGTCTCCCGCCTAGATCGGAAGAG270.3678975337239406Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
GCACACGTCTCATTGCCGATCGGAAGAGCACACGTCTCATTGCCGATCGG260.3542716991415724Illumina Multiplexing Index Sequencing Primer (95% over 22bp)
TCTCCCCACAGATCGGAAGAGCACACGTCTCCCCACAGATCGGAAGAGCA260.3542716991415724Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
CACGTCTCCCGCCTAGATCGGAAGAGCACACGTCTCCCGCCTAGATCGGA260.3542716991415724Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
GACGTGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN240.3270200299768361No Hit
CGTGTGCTCTTCCGATCTAGGCGGGAGACGTGTGCTCTTCCGATCTAGGC240.3270200299768361Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GAAGAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN240.3270200299768361No Hit
TCTCATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN230.31339419539446794No Hit
CGTCTCATTGCCGATCGGAAGAGCACACGTCTCATTGCCGATCGGAAGAG220.29976836081209973Illumina Multiplexing Index Sequencing Primer (95% over 22bp)
CGTGTGCTCTTCCGATCGGCAATGAGACGTGTGCTCTTCCGATCGGCAAT220.29976836081209973TruSeq Adapter, Index 2 (95% over 22bp)
ATCTAGGCGGGAGACGTGTGCTCTTCCGATCTAGGCGGGAGACGTGTGCT210.2861425262297316Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTGGGGAGACGTGTGCTCTTCCGATCTGTGGGGAGACGTGTGCTCTTCCG190.2588908570649952Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGGGGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN190.2588908570649952No Hit
GCAATGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN190.2588908570649952No Hit
GACGTGTGCTCTTCCGATCTAGGCGGGAGACGTGTGCTCTTCCGATCTAG180.24526502248262708Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GATCTAGGCGGGAGACGTGTGCTCTTCCGATCTAGGCGGGAGACGTGTGC180.24526502248262708Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
GATCGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN170.23163918790025886No Hit
GTGCTCTTCCGATCGGAAGAGCACACGTCTCATTGCCGATCGGAAGAGCA170.23163918790025886Illumina Multiplexing Index Sequencing Primer (95% over 22bp)
CTTCCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN170.23163918790025886No Hit
GCACACNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN170.23163918790025886No Hit
CGTCTCCCGCCTAGATCGGAAGAGCACACGTCTCCCGCCTAGATCGGAAG170.23163918790025886Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
GAGCACACGTCTCATTGCCGATCGGAAGAGCACACGTCTCATTGCCGATC160.2180133533178907Illumina Multiplexing Index Sequencing Primer (95% over 22bp)
GATCGGAAGAGCACACGTCTCCCCACAGATCGGAAGAGCACACGTCTCCC160.2180133533178907Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
CTTCCGATCTGTGGGGAGACGTGTGCTCTTCCGATCTGTGGGGAGACGTG160.2180133533178907Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
GAGCACACGTCTCCCGCCTAGATCGGAAGAGCACACGTCTCCCGCCTAGA150.20438751873552258Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
GGAGACGTGTGCTCTTCCGATCTAGGCGGGAGACGTGTGCTCTTCCGATC150.20438751873552258Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCCTAGATCGGAAGAGCACACGTCTCCCGCCTAGATCGGAAGAGCACACG150.20438751873552258Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
AGGCGGGAGACGTGTGCTCTTCCGATCTAGGCGGGAGACGTGTGCTCTTC140.1907616841531544Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCTCCCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN140.1907616841531544No Hit
GGCGGGAGACGTGTGCTCTTCCGATCTAGGCGGGAGACGTGTGCTCTTCC130.1771358495707862Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
AATGAGACGTGTGCTCTTCCGATCGGCAATGAGACGTGTGCTCTTCCGAT130.1771358495707862Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
CACGTCTCATTGCCGATCGGAAGAGCACACGTCTCATTGCCGATCGGAAG130.1771358495707862Illumina Multiplexing Index Sequencing Primer (95% over 22bp)
GCACACGTCTCCCGCCTAGATCGGAAGAGCACACGTCTCCCGCCTAGATC130.1771358495707862Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
ATCTAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN130.1771358495707862No Hit
CTCCCGCCTAGATCGGAAGAGCACACGTCTCCCGCCTAGATCGGAAGAGC120.16351001498841805Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
CGTGTGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN120.16351001498841805No Hit
CGTCTCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN110.14988418040604987No Hit
GGAGACNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN110.14988418040604987No Hit
TCATTGCCGATCGGAAGAGCACACGTCTCATTGCCGATCGGAAGAGCACA100.1362583458236817Illumina Multiplexing Index Sequencing Primer (95% over 22bp)
GCAATGAGACGTGTGCTCTTCCGATCGGAAGAGCACACGTCTCATTGCCG90.12263251124131354Illumina Multiplexing Index Sequencing Primer (95% over 22bp)
TCGGAAGAGCACACGTCTCATTGCCGATCGGAAGAGCACACGTCTCATTG90.12263251124131354Illumina Multiplexing Index Sequencing Primer (95% over 22bp)
CCCGCCTAGATCGGAAGAGCACACGTCTCCCGCCTAGATCGGAAGAGCAC90.12263251124131354Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
TGAGACGTGTGCTCTTCCGATCGGCAATGAGACGTGTGCTCTTCCGATCG90.12263251124131354Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
GTGGGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN90.12263251124131354No Hit
GATCTANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN90.12263251124131354No Hit
GGGGAGACGTGTGCTCTTCCGATCTGTGGGGAGACGTGTGCTCTTCCGAT80.10900667665894535Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
TGAGACNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN80.10900667665894535No Hit
TCTCATTGCCGATCGGCAATGAGACGTGTGCTCTTCCGATCGGCAATGAG80.10900667665894535TruSeq Adapter, Index 5 (95% over 22bp)
CGTCTCCCCACAGATCGGAAGAGCACACGTCTCCCCACAGATCGGAAGAG80.10900667665894535Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
GAGCACNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN80.10900667665894535No Hit
CGGGAGACGTGTGCTCTTCCGATCTAGGCGGGAGACGTGTGCTCTTCCGA80.10900667665894535Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGAGAG450.00398417731.11111370
GGAGAGA500.006670810328.00000269
CGCCTAG800.002377894221.87523
GCCTAGA800.002377894221.87524
CCGCCTA800.002377894221.87522
CTAGATC850.003381598720.58823426
CCTAGAT850.003381598720.58823425
CCCGCCT900.004709989819.44444521
TCTCCCG900.004709989819.44444518
TCCCGCC900.004709989819.44444520
CTCCCGC950.00643967518.42105119
GTCTCCC1000.00865924917.50000217
CGTCTCC1000.00865924917.50000216
CCGATCT1350.00252190115.5555569
TCTTCCG2304.4111392E-412.1739135
CTCTTCC2406.317826E-411.6666664
GCTCTTC2508.908263E-411.2000013
TCCGATC2550.001052030110.9803928
CTTCCGA2600.001238113410.7692316
GTGCTCT2600.001238113410.7692311
Produced by FastQC (version 0.11.2)