FastQCFastQC Report
Fri 3 Aug 2018
H3VNWAFXY_n02_adr3wtzt14s85.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VNWAFXY_n02_adr3wtzt14s85.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences520653
Sequences flagged as poor quality0
Sequence length76
%GC55

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG29300.5627548482386542No Hit
TCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCACAC10580.2032063581694526No Hit
TCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTC10380.19936502814734575No Hit
CTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGC9120.17516464900807255No Hit
CGTCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCAC8450.16229619343401458No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAAG350.00126412939.9404641
ACCTGTA459.1856215E-538.89376441
GTATTAA450.004337027731.064811
TTTCGGA1357.2759576E-1231.0648081
TTGTGTA4550.029.26821970
TAAGCGG500.007249912227.9647735
TATTATT500.007258076727.9583282
ATACAGA808.7260676E-526.2199956
TTCGGAA1900.025.7510912
ATCTGGG4100.025.5804847
TGATACT700.001226900524.99447827
ATTAATT700.00123600924.9627913
TATTAAT700.00123600924.9627912
CTTGTGT5350.024.89166369
CATCAAT851.3163411E-424.6804910
TCAATTA1001.4048057E-524.48046512
CAATTAA1151.5046498E-624.32841113
CGTATAG750.001833551923.32817830
GTTTACT750.001833551923.32817830
TCTGTGT750.001833551923.32817827
Produced by FastQC (version 0.11.2)