FastQCFastQC Report
Fri 3 Aug 2018
H3VNWAFXY_n02_adr3wtzt14s82.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VNWAFXY_n02_adr3wtzt14s82.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences479326
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG11830.2468048885309789No Hit
ATCTAGGACTACCATGGTTGCAACGGGTAACGGGGAATCAGGGTTCGATT8380.17482882213775175No Hit
CCTTAGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCT5820.1214204946111832No Hit
GTCCTAGATACTACCATCAAAAGTTGATAGGGCAGACATTTGAAAGATCT5680.11849972669957398No Hit
GTATAGTACTAACCACAATTGTAAGTTGTACTACCCGTATGAAGCACAAG5390.1124495645969549No Hit
GTTATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACACACGA5170.1078597864501404No Hit
GTACAAGACCATACGATCTGCATGTTATCTAGAGTTCAACCAATATAACG5110.10660802877373646No Hit
GTCTTGTACCGACGACAGATCTTTCAAATGTCTGCCCTATCAACTTTTGA4900.10222687690632264No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTAAAG459.300835E-538.81068813
GATCTGG9000.036.093946
TCTAGGC601.2314451E-534.929628
CCGATCT36650.034.596054
TTCCGAT38500.034.2038082
TCCGATC38550.033.7970123
CGATCTG19450.033.4031335
GATATCA652.1297492E-532.2427251
CTTCCGA42100.031.196051
ATCTGGG4750.030.1497787
ACGTATA703.4857025E-529.99941329
ATCTGGT2100.029.9396747
TCTAGGG1202.3428584E-929.1080172
GACGTAA1452.0008883E-1128.9252718
GATCTAG2300.028.8549046
TAGAGAG854.0129635E-628.765572
CAAGTGT500.00712691828.0624464
CGTAATC1554.3655746E-1127.05912420
GTCCTAG2250.026.3912681
TCTGACC959.525627E-625.7376148
Produced by FastQC (version 0.11.2)