FastQCFastQC Report
Fri 3 Aug 2018
H3VNWAFXY_n02_adr3wtzt14s81.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VNWAFXY_n02_adr3wtzt14s81.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences635479
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG22910.3605154536971324No Hit
ATCTAGGACTACCATGGTTGCAACGGGTAACGGGGAATCAGGGTTCGATT6870.10810742762546047No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCCGAT40400.035.1710972
CCGATCT40150.035.1292344
TCCGATC41600.034.1565483
ATCTGAG2700.032.3318757
GTAGAAC553.035879E-431.738053
CGATCTG21950.031.4981675
CTTCCGA45450.031.3400151
GATCTGG9400.028.603396
TCTAACG500.007282800527.9399958
ATAATAC500.007296194327.9294833
GTATTAA1155.0153176E-827.322321
ATCTAAC658.0628315E-426.8603237
TAGGCCC658.0717E-426.8552724
ATCTGGG4650.026.2826867
CTAGAAG808.8118904E-526.183893
CGATCTA8300.025.6629355
ATATAAG1659.822543E-1125.3904382
CGATCTC10250.024.1873975
TATAAGA1601.9135769E-924.00193
AATAGTG750.001856773223.2789485
Produced by FastQC (version 0.11.2)