FastQCFastQC Report
Fri 3 Aug 2018
H3VNWAFXY_n02_adr3wtzt14s80.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VNWAFXY_n02_adr3wtzt14s80.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences719193
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG13800.1918817341103153No Hit
ATCTAGGACTACCATGGTTGCAACGGGTAACGGGGAATCAGGGTTCGATT9610.13362199020290796No Hit
GTATAGTACTAACCACAATTGTAAGTTGTACTACCCGTATGAAGCACAAG9330.12972873762675666No Hit
GTACTATTACTTTGAACAAATTAGAGTGCTTAAAGCAGGCTTCAAATGCC8620.1198565614515158No Hit
TCGTAATACTAATGCCCCCAAACTGCTTCTATTAATCATTACCTCTTGAT7820.10873298266251201No Hit
GTTATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACACACGA7750.10775966951847417No Hit
GTCTTACGACGGTCCAAGAATTTCACCTCTCGCGTCGTAATACTAATGCC7620.10595208796526107No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATAGG708.811094E-734.938531
TTAATAC751.5108708E-632.6092953
AACCCTA450.00427556231.1574870
TATTATA450.0043440531.056472
ACTATGT500.00726979227.9508231
TTAGCCG808.659632E-526.25190529
ATATAAG3000.025.6215882
AGAGTGT4350.024.97970270
GATCTGG16750.024.406856
AGCGGGA1202.1914984E-623.30203813
ACCGTTA1202.1978467E-623.294298
ATACTGG750.001849954423.2942896
CATATAA3300.023.2923531
ATCTGGG7250.022.651697
ATCTGGC5650.022.2635677
TCTAGGC1102.9584815E-522.2354568
TAGAGGA952.812512E-422.0664392
CACTAGG952.812512E-422.0664391
ACAGATA800.002642727221.90760270
ATGCAAC800.002655549421.88934752
Produced by FastQC (version 0.11.2)