FastQCFastQC Report
Fri 3 Aug 2018
H3VNWAFXY_n02_adr3wtzt14s78.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VNWAFXY_n02_adr3wtzt14s78.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences481927
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG10570.2193278235085397No Hit
ATCTAGGACTACCATGGTTGCAACGGGTAACGGGGAATCAGGGTTCGATT6990.14504271393800305No Hit
TCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCACAC5420.11246516588611968No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGAGTTA350.001254421240.00241534
ATACACT806.319897E-834.9455684
ATATAAG901.7780076E-731.0627272
CATAACA605.024167E-429.121314
AGGTGTA3750.028.06457170
GAGTTAG500.007202747728.00169435
CATAGAA500.00725983227.9564552
TACTACG500.00725983227.9564552
ACTATAC1154.962203E-827.3487073
GTATAAA658.022908E-426.8812071
TAGGTGT4000.026.31053569
TACTATA1251.1131306E-725.160812
GGTCCAA1851.4551915E-1124.5624511
GTACTAT3150.024.406431
ATCTGGG3500.023.9626757
ACGATAT901.9072503E-423.36673762
CGTCGTA3450.023.33474533
ACTGTGT750.00184495223.3028378
TAGGACT2850.023.2970474
ATACCTT1352.3417124E-723.2970476
Produced by FastQC (version 0.11.2)