FastQCFastQC Report
Fri 3 Aug 2018
H3VNWAFXY_n02_adr3wtzt14s76.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VNWAFXY_n02_adr3wtzt14s76.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences454853
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG19680.432667257333688No Hit
ATCTAGGACTACCATGGTTGCAACGGGTAACGGGGAATCAGGGTTCGATT8100.17807951140258502No Hit
CCTTAGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCT5440.11959907926297067No Hit
GTCTTACGACGGTCCAAGAATTTCACCTCTCGCGTCGTAATACTAATGCC5340.117400566776519No Hit
GTTATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACACACGA5260.11564175678735768No Hit
GTCTTGTACCGACGACAGATCTTTCAAATGTCTGCCCTATCAACTTTTGA5240.11520205429006733No Hit
GTCCTAGATACTACCATCAAAAGTTGATAGGGCAGACATTTGAAAGATCT4920.108166814333422No Hit
TCGTAAGACTAACTTAAGCGAAAGCATTTGCCAAAGATGTTTTCATTAAT4730.10398964060916384No Hit
GTATAGTACTAACCACAATTGTAAGTTGTACTACCCGTATGAAGCACAAG4610.10135142562542185No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTATA305.9437635E-446.5556877
CCGATCT25250.037.1984524
TTCCGAT25750.037.154152
TCCGATC26550.035.9031143
TCTAACA501.7324145E-434.9167672
ATATAAG904.598405E-934.9167632
TATAAGA904.598405E-934.9167633
CTTCCGA28500.033.5691031
TAATAAT450.004355322631.0371234
CGATCTG13550.030.4072195
GATCTAG2200.030.1553866
ATCTGAG1401.2732926E-1129.9286587
CGATCTA6300.028.8201875
AATACGC1352.6193447E-1028.47688921
ATCTGGG2950.028.406867
CGAGACT755.6696223E-527.9334134
GATCTGG5800.027.6926086
GTGTAGA1554.1836756E-1127.15005370
TTTAGGG658.0608344E-426.859053
AGACTCA658.0608344E-426.859056
Produced by FastQC (version 0.11.2)