FastQCFastQC Report
Fri 3 Aug 2018
H3VNWAFXY_n02_adr3wtzt14s75.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VNWAFXY_n02_adr3wtzt14s75.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences497759
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG15910.31963259328309485No Hit
GTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACCTGCACGAAT10950.21998597714958443No Hit
TCGTTACGCCATTCGTGCAGGTCGGAACTTACCCGACAAGGAATTTCGCT6330.12716997583167758No Hit
ATCACAAACATTTCAACTTCGCTGAGTCTCGGGAGGAGACAGTGTGGCCA5740.1153168501222479No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTAAC150.002238851769.881871
TTGCGTA753.578316E-837.2792818
GGATACT501.6906371E-435.06300465
CCCTAAT400.002437780434.9409371
GTAGGAA851.07356755E-732.90138622
ACGCTAC751.4666475E-632.72941268
GCGCTTA802.4591209E-630.63215634
TACTTGA500.007137965428.05378368
TGGATAT500.007269081727.9493945
TAGGATA755.648838E-527.9493924
TCTGACC1154.9752998E-827.3417998
TGTAGGG906.250444E-627.1730232
CTAGACA658.0352335E-426.8744164
TGAACGT1056.8668487E-726.61846712
GATACTT808.545321E-526.30042366
AACGCTA808.545321E-526.30042367
ATAAGAG808.764821E-526.2025593
GATCTGG9200.025.822816
GTAAGGC700.001238230224.9548173
TACACCG700.001238230224.9548175
Produced by FastQC (version 0.11.2)