FastQCFastQC Report
Fri 3 Aug 2018
H3VNWAFXY_n02_adr3wtzt14s72.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VNWAFXY_n02_adr3wtzt14s72.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences833514
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG22930.2751003582423331No Hit
GTCTTACGACGGTCCAAGAATTTCACCTCTCGCGTCGTAATACTAATGCC9170.11001614849900541No Hit
GTACTATTACTTTGAACAAATTAGAGTGCTTAAAGCAGGCTTCAAATGCC9120.10941627855081018No Hit
TCGTAATACTAATGCCCCCAAACTGCTTCTATTAATCATTACCTCTTGAT8870.10641692880983403No Hit
GTATTACGACGCGAGAGGTGAAATTCTTGGACCGTCGTAAGACTAACTTA8770.10521718891344357No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCTG32950.033.8300555
TTCCGAT73100.033.4091072
CCGATCT72250.033.076794
TCCGATC74050.032.886133
ATCTGTG2850.031.8783577
CTTCCGA80250.030.5195451
GATCTGG15150.029.9845946
ATCTGGG8400.028.7036577
TCTTTTT6350.026.9643388
TCTGTCC1950.026.8796778
GTGTAAT658.038235E-426.8758221
CTTTTTT6400.025.6616929
CGATCTT17950.025.112665
CGATCTA14950.025.009795
ATCTTTT7100.024.6081587
GGTCCAA3700.024.55671311
GATCTCA5300.024.3945776
GATCTAG4800.024.0237126
TCTGACC1752.055458E-1023.9613138
ATCTGAG3800.023.9087687
Produced by FastQC (version 0.11.2)