FastQCFastQC Report
Fri 3 Aug 2018
H3VNWAFXY_n02_adr3wtzt14s71.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VNWAFXY_n02_adr3wtzt14s71.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences396289
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG32480.8196038749498473No Hit
ATCTAGGACTACCATGGTTGCAACGGGTAACGGGGAATCAGGGTTCGATT7910.19960180575287226No Hit
GTTATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACACACGA5680.14332974167842155No Hit
GTATAGTACTAACCACAATTGTAAGTTGTACTACCCGTATGAAGCACAAG5590.1410586718278832No Hit
CCTTAGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCT5360.13525482665428512No Hit
GTCCTAGATACTACCATCAAAAGTTGATAGGGCAGACATTTGAAAGATCT5020.12667522944114018No Hit
GTCTTGTACCGACGACAGATCTTTCAAATGTCTGCCCTATCAACTTTTGA4870.12289011302357622No Hit
GTACTATTACTTTGAACAAATTAGAGTGCTTAAAGCAGGCTTCAAATGCC4760.12011436098402933No Hit
GTACAAGACCATACGATCTGCATGTTATCTAGAGTTCAACCAATATAACG4760.12011436098402933No Hit
GTCTTACGACGGTCCAAGAATTTCACCTCTCGCGTCGTAATACTAATGCC4400.11103008158187586No Hit
CATATAAGAACTCCACCGGTAATACGCTTACATACATAAAGGTATAGTAC3980.10043175561269678No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGCAC200.00699118252.417273
GTATACT200.006995338552.4093932
TTTCGGA1850.037.7725371
TTCGGAA1850.035.883912
ACTGTGC400.00243574134.9448478
TATACAG501.7237826E-434.9448475
TAATCAC751.5060559E-632.615196
CTGTGCT553.014037E-431.7728169
CTACTAT450.00433744931.0620843
ATACAGT450.00433744931.0620846
TCTATGC450.00433744931.0620848
CCGGGAG1600.030.58133714
TAGACAG802.492112E-630.576745
ATACTGA500.00725878827.9558776
TATAGAC500.00725878827.9558773
ACTTATG906.2269646E-627.18340910
AGATCAT657.9507584E-426.92110832
TACCGAT658.027988E-426.876612
ATCTTTT4300.026.8181387
ATCTGGG2350.026.7662647
Produced by FastQC (version 0.11.2)