FastQCFastQC Report
Fri 3 Aug 2018
H3VNWAFXY_n02_adr3wtzt14s70.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VNWAFXY_n02_adr3wtzt14s70.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences356691
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG24080.6750941290921274No Hit
TCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCACAC4130.11578649307103347No Hit
CTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGC4050.11354365543285365No Hit
TCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTC3830.10737585192785912No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGGTC200.00702026152.3609248
CATATTG200.00702492252.352145
CTAGCGT200.00702492252.352144
CGATTAT350.001266548339.92083415
TAGGCCG350.001271787239.8873445
GGACTGC501.73428E-434.907286
GAATAAG501.735992E-434.9014241
GTGTAGG652.139695E-532.21671
AGTGGAC450.00432747631.0756324
TCTAGGG854.0336636E-628.7423522
ACGCGTC500.007135787528.05293350
GACTGAA755.6178662E-527.9680723
TAGAGCC500.00725999227.95397218
CTAACCT755.682539E-527.921144
CTAGGCC500.00730177127.9211394
TACACCA500.00730177127.9211395
ATCTGGG4150.027.7575997
GACATTT657.9437037E-426.9240535
ATCTGTG1456.0936145E-1026.4813887
TTATCTA808.7168766E-526.22006225
Produced by FastQC (version 0.11.2)