FastQCFastQC Report
Fri 3 Aug 2018
H3VNWAFXY_n02_adr3wtzt14s68.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VNWAFXY_n02_adr3wtzt14s68.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences662058
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG55870.8438837684915823No Hit
TCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCACAC8610.13004902893704176No Hit
TCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTC7810.11796549547018538No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGAG652.1231426E-532.260571
GTATACG652.1231426E-532.260571
TAAGGAT450.00433741431.0657374
TAAGACA450.00433741431.0657374
CGTAAAT1151.4679244E-930.41239423
CTCCTAG604.981317E-429.16632830
ACTGTCC605.021066E-429.126768
AAATTAC1253.5634002E-927.99206726
TCTTAGG500.007258718427.9591622
TAGGCAT500.007258718427.9591625
TATACGG500.007258718427.9591622
CGCGTAA1401.1998054E-824.97708921
GTATGTA851.3176017E-424.67877416
TAGACAG1001.4129431E-524.4642665
GCGCGTA1601.880835E-924.03827320
ATGTATG901.9426893E-423.30562414
TTCGGAA1652.6993803E-923.2993012
ATAACTA750.001847453223.2993015
CTCGGAC1253.0633219E-622.42004253
TAATACG1253.126579E-622.3673292
Produced by FastQC (version 0.11.2)