FastQCFastQC Report
Fri 3 Aug 2018
H3VNWAFXY_n02_adr3wtzt14s67.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VNWAFXY_n02_adr3wtzt14s67.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences524176
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG12510.23866029730472207No Hit
ATCTAGGACTACCATGGTTGCAACGGGTAACGGGGAATCAGGGTTCGATT5830.11122218491499039No Hit
TCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCACAC5720.1091236531241415No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTATA450.00434814231.0486261
GTATATC450.00434814231.0486261
TTAGACT605.038255E-429.108094
GGTCCAA1601.8189894E-1228.38362511
CCTAGGT500.00714370628.0494865
ACCCCTA500.007155710428.03983753
TCTAGCG755.652843E-527.9469578
TCTAACG500.0072766327.9437643
AGAAACG2150.027.72650167
CTAGGAC2950.027.233333
TCTAGGA3400.026.710952
GGAATCA2900.026.54994434
TAGGACT2900.026.4983964
GAGAAAC2250.026.49421366
TTCCGGA3000.025.70023749
AGGACTA3000.025.6151185
GGGTAAC2900.025.32284425
GGTAACG2950.024.89364626
TAACGGG3000.024.4843528
AACGGGT3150.024.41486722
Produced by FastQC (version 0.11.2)