FastQCFastQC Report
Fri 3 Aug 2018
H3VNWAFXY_n02_adr3wtzt14s66.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VNWAFXY_n02_adr3wtzt14s66.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences208392
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG11550.5542439249107451No Hit
TCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCACAC2250.10796959576183347No Hit
CGTCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCAC2100.10077162271104458No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGTC200.007015143552.361525
AGAATCA200.007015143552.361525
GCTAGCT404.6116802E-543.69695325
TTTACTC350.00126923539.894493
TTACTCG350.00126923539.894494
AGGTAAT400.002391021735.06780651
TGCTAGC501.7167744E-434.95755824
TACTAAT501.7312546E-434.9076777
GAGGTAA450.004258025431.1713850
TAGGGGT450.004354513731.0290474
TCTGCTA450.004354513731.0290478
GCATCGT604.9692433E-429.16463933
CTAGCTG605.00265E-429.13129826
TACTCGT500.007287313727.9261425
GGACAGC500.007287313727.9261426
GTTGGAT500.007287313727.9261423
GATCGAA657.790535E-427.00620569
ACCAATG657.84297E-426.97523557
ATACTAA658.055627E-426.852066
ATCTTAC658.055627E-426.852067
Produced by FastQC (version 0.11.2)