FastQCFastQC Report
Fri 3 Aug 2018
H3VNWAFXY_n02_adr3wtzt14s65.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VNWAFXY_n02_adr3wtzt14s65.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences199100
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTCCGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN13150.6604721245605224No Hit
GTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACCCGCACGAAA4010.20140632847815168No Hit
GTGCTCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN3830.19236564540431944No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3130.15720743345052737No Hit
CGTTGAGACAGTGCCCAGATCGTTACGCCTTTCGTGCGGGTCGGAACTTA2640.13259668508287292No Hit
CCTTAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN2250.11300853842290307No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCACGC252.398488E-455.93678
TAACTCA200.006907173452.56622766
ATCTAGT200.006981077652.4250037
ATCTTCG200.006981077652.4250037
GTCTTAG200.006981077652.4250037
CTAATAG200.006981077652.4250033
ATCTGGC802.910383E-1143.68757
GAACGTG350.001259685639.95478411
AAACTAT350.001261537239.9428566
CTGGACT350.001261537239.9428564
CGATCTA3200.037.1343775
GTGTAGA501.6886869E-435.0546470
CGATCAA501.697629E-435.02318243
GGTAGCA501.7006183E-435.01270739
CGATTTA400.00241987734.9813326
TGGGCTC400.00243054134.955
GATCTTC400.00243054134.956
GTAAACT501.7186461E-434.954
GATCTAG1350.033.6555566
TTTACCG751.4844863E-632.65899730