FastQCFastQC Report
Fri 3 Aug 2018
H3VNWAFXY_n02_adr3wtzt14s64.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VNWAFXY_n02_adr3wtzt14s64.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences779275
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG17600.22585095120464535No Hit
ATCTAGGACTACCATGGTTGCAACGGGTAACGGGGAATCAGGGTTCGATT14250.1828622758333066No Hit
GTATAGTACTAACCACAATTGTAAGTTGTACTACCCGTATGAAGCACAAG11730.15052452600173238No Hit
GTTATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACACACGA11550.1482146867280485No Hit
GTCCTAGATACTACCATCAAAAGTTGATAGGGCAGACATTTGAAAGATCT10710.13743543678419043No Hit
GTCTTGTACCGACGACAGATCTTTCAAATGTCTGCCCTATCAACTTTTGA9810.12588624041577107No Hit
CCTTAGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCT9630.12357640114208719No Hit
GTCTTACGACGGTCCAAGAATTTCACCTCTCGCGTCGTAATACTAATGCC9480.12165153508068398No Hit
GTACTATTACTTTGAACAAATTAGAGTGCTTAAAGCAGGCTTCAAATGCC8990.11536363928010009No Hit
GTACAAGACCATACGATCTGCATGTTATCTAGAGTTCAACCAATATAACG8870.11382374643097752No Hit
TCGTAATACTAATGCCCCCAAACTGCTTCTATTAATCATTACCTCTTGAT7860.10086298161752913No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGACA605.041712E-429.1072964
CCTTACA1352.6557245E-1028.460472
TTATAGG959.5392825E-625.7369793
TTACTCA700.00123896124.9548711
TCTAGGG1401.2150849E-824.9491122
GGTCCAA3150.024.4003211
GATCTGG13800.023.2876286
TATAGGT750.001854022323.285844
GTATAAA901.9544149E-423.285841
AGTGTAG3350.023.03398569
GTCCTAG5350.022.850591
CTTACAC2450.022.8106173
CTTGGGG2300.022.7796253
GGTACAA5100.022.6794857
GTGTAGA3450.022.3663370
TTTCGGA2350.022.2949521
TCTAGGC952.8152432E-422.0636628
ATACAGC952.816709E-422.0619646
GTATTAA952.8181754E-422.0602681
GTATATA800.002697630821.8304731
Produced by FastQC (version 0.11.2)