FastQCFastQC Report
Fri 3 Aug 2018
H3VNWAFXY_n02_adr3wtzt14s63.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VNWAFXY_n02_adr3wtzt14s63.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences375356
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG17790.4739500634064728No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTTATG305.892101E-446.63559323
TCTTGCG951.891749E-1036.7948
GTGCTTG601.2246299E-534.95439
AGTTATG400.002432284434.95438
TTAATAC400.002436107534.943113
TCGTGGG601.2273194E-534.943112
AGGCTTA552.9861246E-431.82248138
ACTAGTT553.0171566E-431.7664663
CGTAATA450.004304227431.11032736
ATGAGGG450.004331309331.0704867
GTGTTAT450.00433810131.0605431
GATCTGG10350.030.057326
AAAGAGT703.521683E-529.9512394
CGGTGTA3100.029.39650770
TATTACT605.0142896E-429.1285825
GTGTAGG853.997173E-628.776681
GTACTAG853.997173E-628.776681
GTTGCGA500.007158339528.03524261
AATACTA500.00719769428.00378439
CCTGCTA500.0072033327.99929431
Produced by FastQC (version 0.11.2)