FastQCFastQC Report
Fri 3 Aug 2018
H3VNWAFXY_n02_adr3wtzt14s62.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VNWAFXY_n02_adr3wtzt14s62.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences239670
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG13130.5478366086702549No Hit
ATCTAGGACTACCATGGTTGCAACGGGTAACGGGGAATCAGGGTTCGATT5170.21571327241623897No Hit
GTATAGTACTAACCACAATTGTAAGTTGTACTACCCGTATGAAGCACAAG3630.15145825510076355No Hit
GTCTTGTACCGACGACAGATCTTTCAAATGTCTGCCCTATCAACTTTTGA3330.13894104393541118No Hit
TCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCACAC3260.1360203613301623No Hit
CCTTAGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCT3120.13017899611966452No Hit
CTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGC3070.12809279425877249No Hit
TCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTC2980.12433763090916676No Hit
GTTATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACACACGA2720.11348938123252805No Hit
CGTCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCAC2680.11182041974381442No Hit
GTCCTAGATACTACCATCAAAAGTTGATAGGGCAGACATTTGAAAGATCT2670.11140317937163599No Hit
GTCTTACGACGGTCCAAGAATTTCACCTCTCGCGTCGTAATACTAATGCC2520.10514457378895982No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACAGA350.001264840539.9254386
TAAGCGA654.885933E-737.65022715
AATCTTT501.6916313E-435.04843562
TTACTCA400.002436887234.9347534
TATTGGG400.002436887234.9347532
CTTTGCC400.002436887234.9347539
AGGTTCG751.4677389E-632.71187267
CATTAAT652.0899239E-532.3200444
TTGCCAA652.1006765E-532.29581528
CCTACTC450.004339499431.0531168
AGAGGTT703.4439097E-530.04151765
TTAAGCG703.5092053E-529.95902614
ATGGCTT604.9296685E-429.2070355
TCTTTGA604.9296685E-429.2070364
TGGCTTA604.9296685E-429.2070356
TTGGGCA605.0243724E-429.11229710
TGGAAAT500.00714792128.03874856
AAAGTTA755.5131313E-528.03874859
GTGGGGT500.007262238327.9478054
TAGATAC1253.594323E-927.9478035
Produced by FastQC (version 0.11.2)