FastQCFastQC Report
Fri 3 Aug 2018
H3VNWAFXY_n02_adr3wtzt14s61.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VNWAFXY_n02_adr3wtzt14s61.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences426794
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG16650.39011794917454323No Hit
ATCTAGGACTACCATGGTTGCAACGGGTAACGGGGAATCAGGGTTCGATT6600.15464134922234146No Hit
GTATAGTACTAACCACAATTGTAAGTTGTACTACCCGTATGAAGCACAAG5330.12488460475076968No Hit
GTCTTGTACCGACGACAGATCTTTCAAATGTCTGCCCTATCAACTTTTGA4950.11598101191675608No Hit
GTTATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACACACGA4820.11293504594722512No Hit
TCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCACAC4510.10567158863526666No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACTA200.006937341852.52058437
CGGTATA200.006972163452.4541420
AGAAACG2100.036.72239367
TCTGAGT553.0171068E-431.768068
CCTTGTA553.0171068E-431.768062
TAGGACA450.0043378431.0621014
ATATAAG1301.6916601E-1029.5687332
TTGTCTC1800.029.1207210
ACCGGTA1451.8189894E-1128.93614615
TCGATTC2750.028.01492145
TACTTGA500.007189999328.01097942
GGAGCCT2900.027.78514759
CTTGTCT1900.027.5880539
TCAGGGT2800.027.51078238
GAGAAAC2950.027.32960566
GGTTGCA3100.027.06929816
GGAATCA3000.026.83252534
TAGGACT3000.026.7910634
CATACAT1455.9117156E-1026.55092431
TACATAC1455.9299055E-1026.54345329