FastQCFastQC Report
Fri 3 Aug 2018
H3VNWAFXY_n02_adr3wtzt14s5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VNWAFXY_n02_adr3wtzt14s5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1542775
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCACAC70170.45482977102947614No Hit
TCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTC62700.4064105264863639No Hit
CTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGC58930.3819740402845522No Hit
CGTCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCAC53560.3471666315567727No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG31080.20145517006692487No Hit
CGTCTAGATCGGAAGAGCACACGTCTAGATCGGAAGAGCACACGTCTAGA27990.18142632593864952Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
ATCTAGACGTGTGCTCTTCCGATCTAGACGTGTGCTCTTCCGATCTAGAC23980.15543420135794267Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
GAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTC16450.10662604722010663No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATAGCA1301.6370905E-1029.67463369
GTATTAA605.0416077E-429.1100711
TACCTAT1352.6375346E-1028.46983310
ACATAAT2450.027.19715969
AGTATCG808.568012E-526.29634967
TTTCGGA2550.026.0278281
GATACTC77950.023.39501270
CGAGTAA1652.6011548E-923.38001470
ATGTTAT3450.023.31165922
TAGCCCA901.9448198E-423.305319
TCCATAA750.001853976323.2880552
TGCACAA3200.022.93757816
TCGATTC4300.022.81043245
GAAACTA1102.879016E-522.31728670
GGACTGC1102.9644169E-522.2329716
ATAGCAA1905.493348E-1022.14948770
ATACCTA1454.6859168E-721.687059
TTGTGTA11800.021.39865770
TGTAAGG1154.182146E-521.2630082
GTATAGG3000.020.959251
Produced by FastQC (version 0.11.2)