Basic Statistics
Measure | Value |
---|---|
Filename | H3VNWAFXY_n02_adr3wtzt14s59.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 396164 |
Sequences flagged as poor quality | 0 |
Sequence length | 76 |
%GC | 54 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG | 2419 | 0.6106057087468826 | No Hit |
TCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCACAC | 660 | 0.16659767167132802 | No Hit |
CTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGC | 555 | 0.14009349663270768 | No Hit |
TCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTC | 550 | 0.13883139305944003 | No Hit |
CGTCTAGATCGGAAGAGCACACGTCTAGATCGGAAGAGCACACGTCTAGA | 467 | 0.11788047374319725 | Illumina Multiplexing PCR Primer 2.01 (95% over 21bp) |
CGTCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCAC | 451 | 0.11384174230874083 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
TATAGCA | 25 | 2.4124786E-4 | 55.887062 | 3 |
ATTTACT | 20 | 0.006999172 | 52.40205 | 7 |
TATTCGA | 20 | 0.0070033623 | 52.39412 | 2 |
GAGTCTT | 35 | 0.0012585216 | 39.973766 | 25 |
TATGCTT | 35 | 0.0012660422 | 39.92537 | 5 |
ACACAAT | 65 | 4.7948197E-7 | 37.753387 | 67 |
CACAATA | 80 | 6.131813E-8 | 35.062042 | 68 |
GTCTAGT | 40 | 0.002412142 | 35.01418 | 40 |
AGCATAC | 40 | 0.0024355685 | 34.945274 | 10 |
CGCTAAT | 50 | 1.723631E-4 | 34.945274 | 14 |
GTTATAT | 40 | 0.002441 | 34.929413 | 3 |
TAAAATC | 45 | 0.0042796023 | 31.147339 | 51 |
GAGATAC | 45 | 0.004311493 | 31.10013 | 31 |
ATATGCT | 45 | 0.00434679 | 31.04837 | 4 |
TTATAGG | 45 | 0.00434679 | 31.04837 | 4 |
TAGTCGC | 50 | 0.0071781566 | 28.019844 | 43 |
GAGCATA | 50 | 0.0072743855 | 27.943531 | 1 |
CTACACT | 100 | 4.487083E-7 | 27.943531 | 4 |
ACGTGAT | 65 | 7.865412E-4 | 26.970802 | 69 |
CACACAA | 65 | 7.872396E-4 | 26.966705 | 66 |