FastQCFastQC Report
Fri 3 Aug 2018
H3VNWAFXY_n02_adr3wtzt14s58.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VNWAFXY_n02_adr3wtzt14s58.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences210194
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACCTGCACGAAT4600.21884544753894022No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3840.18268837359772402No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAAAGC150.002235701369.889716
CCGCATT252.3872676E-455.9913529
ATCCGAC200.00693089852.52178643
CTGGCCG352.0996944E-549.9212239
ATCTGGC703.8744474E-1044.92917
ATCTGGG1750.041.933837
GATCTTT602.615252E-740.7689976
GATCTGG3450.039.502886
CGGGAAC901.05501385E-1038.84970515
TTAGGTG556.647888E-638.19766645
TCTGTGC556.7394612E-638.1216628
CGATCTG10350.037.1394585
CGATCTA5650.037.109585
CGCGGTG400.002408965335.01452652
TCTGCAG400.00242584934.9647310
CGGGTGT400.00242584934.9647315
TTCCGAT24900.034.944862
TCTGGCC501.7204844E-434.944858
TCCGATC25400.034.2569663
CCGATCT25400.034.2569664
Produced by FastQC (version 0.11.2)