FastQCFastQC Report
Fri 3 Aug 2018
H3VNWAFXY_n02_adr3wtzt14s57.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VNWAFXY_n02_adr3wtzt14s57.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences225667
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTCCGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN15700.6957153682195447No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG11060.4901026734081633No Hit
GTGCTCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN6740.29867016444584277No Hit
CGTCTCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN5490.2432788134729491No Hit
ATCTAGGACTACCATGGTTGCAACGGGTAACGGGGAATCAGGGTTCGATT5060.22422418873827366No Hit
GTATAGTACTAACCACAATTGTAAGTTGTACTACCCGTATGAAGCACAAG4160.1843424160377902No Hit
GAAGAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN4150.18389928523000704No Hit
GTCTTGTACCGACGACAGATCTTTCAAATGTCTGCCCTATCAACTTTTGA3540.15686830595523493No Hit
GTTATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACACACGA3390.15022134383848768No Hit
GATCGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN2920.12939419587267967No Hit
GTCTTACGACGGTCCAAGAATTTCACCTCTCGCGTCGTAATACTAATGCC2800.12407662617928186No Hit
GTCCTAGATACTACCATCAAAAGTTGATAGGGCAGACATTTGAAAGATCT2780.12319036456371556No Hit
GTACTATTACTTTGAACAAATTAGAGTGCTTAAAGCAGGCTTCAAATGCC2760.12230410294814925No Hit
CCTTAGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCT2690.1192021872936672No Hit
GTACTAACCACAATTGTAAGTTGTACTACCCGTATGAAGCACAAGTTCAA2670.11831592567810092No Hit
GAGCACNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN2630.11654340244696831No Hit
TCGTAATACTAATGCCCCCAAACTGCTTCTATTAATCATTACCTCTTGAT2460.10901017871465478No Hit
CATATAAGAACTCCACCGGTAATACGCTTACATACATAAAGGTATAGTAC2410.10679452467573904No Hit
GTACAAGACCATACGATCTGCATGTTATCTAGAGTTCAACCAATATAACG2320.10280634740569068No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA2280.1010338241745581No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGAAAA200.00688608852.60950562
TGGTAAA200.006965979552.45632625
TCCAGGA200.00699520152.4008452
GTCTTAA200.00699520152.4008451
ACGTATG501.7187916E-434.9523821
TTTCGGA501.7241474E-434.93391
CAGGGGT501.7241474E-434.93394
CCGAGCG400.002436834234.93399
GTTCAGG501.7241474E-434.93391
GTCCTAG1400.032.4386181
TGTTAAC450.00428908731.12642338
GGTAAAT450.00431698631.08522826
GAATTGC450.00431698631.08522827
GTAAATC450.00431698631.08522827
CATACAA450.004333794631.06056414
GGGAGTC450.004339408631.0523517
AGAAACG1551.8189894E-1229.40825867
AGACGTA605.008494E-429.12698619
CACGGGG605.024036E-429.11158212
CCTCAGG605.024036E-429.1115821
Produced by FastQC (version 0.11.2)