FastQCFastQC Report
Fri 3 Aug 2018
H3VNWAFXY_n02_adr3wtzt14s56.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VNWAFXY_n02_adr3wtzt14s56.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences671505
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG75411.1229998287428984No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGACTA350.001266332639.9283335
CTAGGAC556.7800793E-638.1100043
GATCTTT5100.036.9924286
CGATCTG26200.035.870735
TTCCGAT60100.034.9341742
TATATGC400.002440810734.934174
CCGATCT59150.034.904644
TCCGATC60800.034.4170533
CGATCTT12550.034.2413335
CTAGGCG751.5110381E-632.608149
GATCTAG4400.032.5552066
ATTATGG553.024723E-431.7583373
CTCTATA450.00434644531.0525951
CTTCCGA68000.030.875641
TCTGTCC802.5003337E-630.570138
TAGGACT802.5020781E-630.5673984
TCTTTTT4750.030.15648
TGTATAC703.53278E-529.9435733
CTTTTTT4950.028.937969
ATCTTTT5450.028.8473077
Produced by FastQC (version 0.11.2)