FastQCFastQC Report
Fri 3 Aug 2018
H3VNWAFXY_n02_adr3wtzt14s55.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VNWAFXY_n02_adr3wtzt14s55.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences393095
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG21540.5479591447360054No Hit
ATCTAGGACTACCATGGTTGCAACGGGTAACGGGGAATCAGGGTTCGATT11600.2950940612320177No Hit
GTATAGTACTAACCACAATTGTAAGTTGTACTACCCGTATGAAGCACAAG9760.24828603772624938No Hit
GTCTTGTACCGACGACAGATCTTTCAAATGTCTGCCCTATCAACTTTTGA7990.20325875424515705No Hit
GTTATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACACACGA7830.19918849133161196No Hit
GTCCTAGATACTACCATCAAAAGTTGATAGGGCAGACATTTGAAAGATCT7260.18468817970210763No Hit
CCTTAGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCT6880.1750213052824381No Hit
GTACTATTACTTTGAACAAATTAGAGTGCTTAAAGCAGGCTTCAAATGCC6540.1663719965911548No Hit
GTCTTACGACGGTCCAAGAATTTCACCTCTCGCGTCGTAATACTAATGCC6320.16077538508503034No Hit
GTACAAGACCATACGATCTGCATGTTATCTAGAGTTCAACCAATATAACG6210.1579770793319681No Hit
GTACTAACCACAATTGTAAGTTGTACTACCCGTATGAAGCACAAGTTCAA6140.15619633930729213No Hit
CATATAAGAACTCCACCGGTAATACGCTTACATACATAAAGGTATAGTAC5690.1447487248629466No Hit
GGATAATGAAATAAGACCTCTGTTCTGCTTTCATTGGTTTTCAGATCAAG5340.13584502473956678No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA5150.131011587529732No Hit
CCTTATGGGACATGTGCTTTTATTAGGCTAAAACCAAGCGATCGCAAGAT5130.13050280466553887No Hit
GTATTACGACGCGAGAGGTGAAATTCTTGGACCGTCGTAAGACTAACTTA5040.12821328177666974No Hit
CATCTAAGGAAGGCAGCAGGCGCGTAAATTACCCACTCCCAGCTCGGGGA4920.12516058459151094No Hit
TCGTAATACTAATGCCCCCAAACTGCTTCTATTAATCATTACCTCTTGAT4900.12465180172731782No Hit
TCGTAAGACTAACTTAAGCGAAAGCATTTGCCAAAGATGTTTTCATTAAT4810.12236227883844872No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGAGCTGGGAGTGGGTA4580.11651127590022768No Hit
GCTTAAAGCAGGCTTCAAATGCCTGAATATTCTGTGCATGGGATAATGAA4550.11574810160393798No Hit
GTACTACCCGTATGAAGCACAAGTTCAACTACGAACGTTTTAACCGCAAC4470.11371297014716544No Hit
GATATGAGTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGAGCTGGGA4390.11167783869039291No Hit
TTCTAGAGCTAATACATGCAATTAAAACATGAACCTTATGGGACATGTGC4380.11142344725829634No Hit
CTTTAAATCCTTTAACAAGGACCAATTGGAGGGCAAGTCTGGTGCCAGCA4250.10811635864104097No Hit
ATACAGGACTCATATCCGAGGCCCTGTAATTGGAATGAGTACACTTTAAA4110.10455487859168902No Hit
GAACTAGGGCGGTATCTGATCGCCTTCGAACCTCTAACTTTCGTTCTTGA4100.10430048715959248No Hit
CATAAAGGTATAGTACTAACCACAATTGTAAGTTGTACTACCCGTATGAA4030.1025197471349165No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTCTA200.00696112952.474329
CTAGCAC200.0069736652.4504473
TGTCAAC400.00242095134.98817436
TCTAGTA400.00242816434.9669652
TCAGAAC601.2218023E-534.9669653
TACACGA400.00242816434.9669652
GGCTAAC501.717353E-434.9669651
GATTATG802.4797646E-630.5960947
GGTCCAA2600.029.59191511
TCTGAGC1301.6734703E-1029.5874318
TTCAGAA605.0042704E-429.139142
CCTTGAG605.0042704E-429.139142
AGAAACG4300.027.70707967
TATGCTT3300.027.5497285
TACTATA1403.9835868E-1027.4740452
CTTGGGT657.99121E-426.8976633
GTAAATT2350.026.79132824
AAACGGC4450.026.777269
AACGGCT4500.026.47967370
GAGAAAC4500.026.47163266
Produced by FastQC (version 0.11.2)