FastQCFastQC Report
Fri 3 Aug 2018
H3VNWAFXY_n02_adr3wtzt14s54.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VNWAFXY_n02_adr3wtzt14s54.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences221717
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG17290.779822927425502No Hit
TCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTC2470.11140327534650027No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGTCC305.9286784E-446.567098
TTTCGGA459.332577E-538.7745441
GGAGCGA501.7210416E-434.9441617
CCATTAT501.7319931E-434.9064982
TATATGC601.2334656E-534.9064943
GCACTGT652.1253969E-532.2387546
TGCTTGC450.004344412631.04472510
ATCAATG450.004344412631.0447257
CATGTTA703.5068482E-529.96021721
ACCAATA604.951963E-429.18311139
TTTCGGG500.007203675327.99308433
AGACTAA500.007270444727.9402546
GGAACCC755.6447607E-527.9402525
TAAATAC500.007289611327.9251983
GCCTAGG500.007299209527.9176731
CGAATTA657.8704726E-426.96012348
ACGAATT657.8829285E-426.95283147
TAGAGTT657.958007E-426.90915930
TTATCTA657.983164E-426.89463225
ACCCCAC658.033674E-426.865638
Produced by FastQC (version 0.11.2)