FastQCFastQC Report
Fri 3 Aug 2018
H3VNWAFXY_n02_adr3wtzt14s50.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VNWAFXY_n02_adr3wtzt14s50.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences230312
Sequences flagged as poor quality0
Sequence length76
%GC55

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG27581.1975059918718955No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACACTA150.00223036169.9344920
GTCTAGG252.4032472E-455.918631
GTCGTAC200.006983367752.4237171
TAAGACC200.006983367752.4237174
TTGTGAG305.901862E-446.61092413
AGACTTG305.9093995E-446.5988585
GATTTTG350.001258958739.96256623
CCTAGGG350.001262169339.9418832
TACTAGT556.7372257E-638.1263432
GTGTAGA1600.037.2394870
AGTGTAG1650.036.1110169
ACTCCTA708.5734246E-735.0398357
TACTGGA400.00240408335.0307440
TACTCCG400.002425582834.96724325
AGGACAG400.002431753634.9491465
GTACTAG601.2234515E-534.9491461
CTGCACT400.002431753634.9491464
AGACACG751.4761736E-632.6868844
TTAACGC553.0007403E-431.78840422
TCCGATT553.0053037E-431.78017617
Produced by FastQC (version 0.11.2)