FastQCFastQC Report
Fri 3 Aug 2018
H3VNWAFXY_n02_adr3wtzt14s4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VNWAFXY_n02_adr3wtzt14s4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1200919
Sequences flagged as poor quality0
Sequence length76
%GC55

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCACAC57670.4802155682439865No Hit
TCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTC51240.4266732394108179No Hit
CTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGC49990.4162645440699997No Hit
CGTCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCAC44050.3668024238104319No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG24090.20059637660824753No Hit
CGTCTAGATCGGAAGAGCACACGTCTAGATCGGAAGAGCACACGTCTAGA21270.17711435991936175Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
ATCTAGACGTGTGCTCTTCCGATCTAGACGTGTGCTCTTCCGATCTAGAC19800.1648737341985596Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
GAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTC13080.10891658804632119No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGT252.4121963E-455.899521
TCAGAGT305.831323E-446.74387766
AATTGTA601.2043405E-535.05088861
TGTTATA400.002412739435.0193345
TTTCGGA2200.034.9372021
GACACAT851.04942956E-732.99567866
CATATGC1750.030.0541570
ATTGTAT604.937281E-429.2134662
AAGTACG605.024751E-429.12596115
TTGCACA1202.3355824E-929.12596115
ACATAAT500.00714672928.0505469
TGTATAC1004.3601722E-728.0463364
ACTCATT500.00718876328.01686746
TTATGTA500.007273415627.949763
ATATGCC906.0956663E-627.26726365
TGCACAA2200.027.01175316
TTAGGAT657.8913395E-426.96222360
AGTACGT658.016789E-426.8895316
TAATGAT658.0425444E-426.874774
ACATATG2100.026.71480269
Produced by FastQC (version 0.11.2)