FastQCFastQC Report
Fri 3 Aug 2018
H3VNWAFXY_n02_adr3wtzt14s49.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VNWAFXY_n02_adr3wtzt14s49.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences452734
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG30340.6701506845079008No Hit
GTCCTGGGCTGCACGCGCGCTACAATGAAAGTATCAACGTGTATTTCCTA5160.11397421002177878No Hit
GTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACCTGCACGAAT4740.10469723943861076No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGAGT556.7678066E-638.115338
TTCCGAT47850.036.71832
CGATCTG21700.036.2223475
CCGATCT48350.035.9821134
TCCGATC48950.035.8218083
CCACTAG400.002403627135.04075257
AAACACT400.002436572234.9436616
TCTTGTC400.0024381534.939058
TAGACTG400.002439728534.934445
GTGTTAA501.7285957E-434.9298321
TCTAGAC806.343362E-834.9298323
CGATCTA9000.034.158125
CTTCCGA53500.032.905861
TAATATT652.1274245E-532.2471774
CGATCTT8700.032.1236235
CTAGGAC553.0250993E-431.7543933
GATCTGG10600.031.309176
GATCTTG3200.030.5676366
ATCTGGT1850.030.213577
CGATCTC12900.029.7890595
Produced by FastQC (version 0.11.2)