FastQCFastQC Report
Fri 3 Aug 2018
H3VNWAFXY_n02_adr3wtzt14s48.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VNWAFXY_n02_adr3wtzt14s48.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences500729
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG21490.4291742639232No Hit
ATCTAGGACTACCATGGTTGCAACGGGTAACGGGGAATCAGGGTTCGATT10320.2060995069189122No Hit
CCTTAGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCT6620.13220724184139523No Hit
GTCCTAGATACTACCATCAAAAGTTGATAGGGCAGACATTTGAAAGATCT6450.12881219182432013No Hit
GTCTTACGACGGTCCAAGAATTTCACCTCTCGCGTCGTAATACTAATGCC6110.12202209179016993No Hit
GTTATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACACACGA6110.12202209179016993No Hit
GTCTTGTACCGACGACAGATCTTTCAAATGTCTGCCCTATCAACTTTTGA5480.10944043584453866No Hit
GTATAGTACTAACCACAATTGTAAGTTGTACTACCCGTATGAAGCACAAG5440.10864160054640334No Hit
GTATTACGACGCGAGAGGTGAAATTCTTGGACCGTCGTAAGACTAACTTA5420.10824218289733568No Hit
GTACAAGACCATACGATCTGCATGTTATCTAGAGTTCAACCAATATAACG5350.10684422112559888No Hit
TCGTAAGACTAACTTAAGCGAAAGCATTTGCCAAAGATGTTTTCATTAAT5260.1050468417047944No Hit
GTACTATTACTTTGAACAAATTAGAGTGCTTAAAGCAGGCTTCAAATGCC5050.100852956389584No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCCGAT33250.034.873282
TCCGATC33950.034.1542433
CCGATCT33800.033.9958234
CTTCCGA36650.031.6381051
CGATCTG17750.030.1050535
GATCTGG7550.029.1433836
TCTAGGG605.041132E-429.1048342
GTTTAGA755.6608907E-527.940641
GTCATAG500.007280197527.9406381
GTACATG500.007280197527.9406381
ATTGGGC500.007280197527.9406383
GATCTAG3000.026.7764476
TCTAGGC808.78349E-526.194358
ATCTAGC959.534782E-625.7347987
ACAGGGA959.534782E-625.73479813
ATCTGGG3400.025.6807357
CGATCTC10350.025.6459985
TCTGGTC1251.11944246E-725.1465768
ATCTCGT1551.3169483E-924.786057
TCAAGTC851.2981468E-424.73077248
Produced by FastQC (version 0.11.2)