FastQCFastQC Report
Fri 3 Aug 2018
H3VNWAFXY_n02_adr3wtzt14s45.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VNWAFXY_n02_adr3wtzt14s45.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences328688
Sequences flagged as poor quality0
Sequence length76
%GC55

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8940.27199045903714164No Hit
TCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCACAC5520.16794041766051698No Hit
CTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGC4180.12717227279365234No Hit
CGTCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCAC4020.12230443460059387No Hit
TCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTC3970.12078323516526311No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACTAA150.002211887670.0891164
TTACTCG200.006997288752.403164
GGCCTAG400.002399006935.05099570
ATTTTAT400.002401170835.0445563
ACTAGTA400.002438194834.935442
CATTTTA450.00427604131.15071562
CGTGTTT1401.2732926E-1129.97760418
CTAGTAC703.525351E-529.9446623
TAAGGCT605.028512E-429.1128674
GCAGAGT1451.8189894E-1128.94389325
TTCGTGT1451.8189894E-1128.93858716
TTTTTAT755.5592358E-528.00991238
TCGTGTT1502.910383E-1127.97396917
TCAGGTT500.007259587827.9534726
TTAGAGC500.007266083727.9483533
CAGAGTG1403.947207E-1027.4845126
TCTGGGG906.2330346E-627.1769858
GGAACGT657.986976E-426.89804517
TTTCGGA959.498746E-625.7419031
ATCTGGG3200.025.1144477
Produced by FastQC (version 0.11.2)