FastQCFastQC Report
Fri 3 Aug 2018
H3VNWAFXY_n02_adr3wtzt14s44.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VNWAFXY_n02_adr3wtzt14s44.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences347010
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG15620.45013112014062995No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCCGTC459.098302E-538.95130565
TACAGTA400.00241392435.00739342
AATGATC450.00429869531.11768344
GAATATA450.004335419331.0636521
TATCGGT755.625363E-527.96214113
TCTGAGT500.0072492627.9621378
TTCTAAG755.632026E-527.9572852
ATTCTAA500.0072554127.9572831
TCTATAC500.0072554127.9572833
ACCGTTA658.007816E-426.8866758
GGCTAAT658.0159464E-426.8820061
TTACACT808.7284876E-526.2145084
CTATCCT959.464809E-625.7546024
TTCACTA851.317741E-424.6724788
CACTATC851.317741E-424.67247810
TACTGGT851.319296E-424.6681922
AATCCTT1001.4087227E-524.466876
GTGTGTA3050.024.13703770
TAAGTTG1052.0523885E-523.3058317
CTATCGG901.9417115E-423.30178312
Produced by FastQC (version 0.11.2)