FastQCFastQC Report
Fri 3 Aug 2018
H3VNWAFXY_n02_adr3wtzt14s42.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VNWAFXY_n02_adr3wtzt14s42.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences326560
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG21350.6537849093581577No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCTAGG200.00701154652.3761710
CCTTACA551.3360295E-744.4240842
TTTCGGA801.4551915E-939.267721
TACCATA400.002402834435.0395849
CTGCTAG400.002446557934.911049
TAATACT601.2360388E-534.904644
TCCTATA400.002448760434.904641
TTACACT652.137686E-532.219674
ATCTGTG1500.030.2562377
ATCACTC605.049099E-429.0925358
ACTGTAG605.049099E-429.0925358
TCTGTCC854.02337E-628.750278
CTATACT500.007297417627.9237144
CTTACAC1155.0065864E-827.3166753
TCTGTGC906.275859E-627.153038
TCTGGCC1056.896389E-726.5988888
TCTGTGT808.797838E-526.183288
ACCTTAC1101.0365111E-625.3851911
ACTGAAT1251.12106136E-725.135958
TGGTAAC700.001230537724.9776922
Produced by FastQC (version 0.11.2)