FastQCFastQC Report
Fri 3 Aug 2018
H3VNWAFXY_n02_adr3wtzt14s41.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VNWAFXY_n02_adr3wtzt14s41.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences442991
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG18790.42416211616037347No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATATA252.4061279E-455.918631
ATCTGAG2200.031.771957
ATCTAGT901.7757338E-731.0659057
CATAACT500.007193288828.00862734
TCTGAGC1004.4673834E-727.9593148
ATCTAGC2000.027.9593147
GTCAGAC500.007255445727.9593142
TTGCAAG500.007255445727.95931413
CTAGCAG500.007255445727.9593149
GTACTGG1404.0017767E-1027.4600451
TCTGTCA1154.955109E-827.3515078
ACTATAG906.2307117E-627.1826673
TTTCGGA1101.0267086E-625.4175591
TACTAGT700.001235404424.9636762
GTCGCTA851.2943533E-424.74034569
TACTGGT851.3196313E-424.6699852
CGATCTG26300.024.3182265
ACACTAC750.001831733623.33102830
ACTGCTC750.001846235423.299438
TACTATA1052.0587946E-523.299432
Produced by FastQC (version 0.11.2)