FastQCFastQC Report
Fri 3 Aug 2018
H3VNWAFXY_n02_adr3wtzt14s40.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VNWAFXY_n02_adr3wtzt14s40.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences605993
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG43140.7118894112638264No Hit
ATCTAGGACTACCATGGTTGCAACGGGTAACGGGGAATCAGGGTTCGATT11180.18449057992419055No Hit
CCTTAGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCT7730.12755922923202082No Hit
GTCTTGTACCGACGACAGATCTTTCAAATGTCTGCCCTATCAACTTTTGA7350.12128852973549199No Hit
GTCCTAGATACTACCATCAAAAGTTGATAGGGCAGACATTTGAAAGATCT7230.11930830884185131No Hit
GTTATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACACACGA6910.11402771979214281No Hit
GTATAGTACTAACCACAATTGTAAGTTGTACTACCCGTATGAAGCACAAG6690.11039731482046823No Hit
GTCTTACGACGGTCCAAGAATTTCACCTCTCGCGTCGTAATACTAATGCC6340.10462167054734955No Hit
GTACAAGACCATACGATCTGCATGTTATCTAGAGTTCAACCAATATAACG6060.10000115512885462No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTGTA305.9250824E-446.588394
ATATAAG1600.037.1251262
ATCTGGG4300.036.566477
TATAAGA1850.033.9969373
TTCCGAT44900.033.384892
CCGATCT44700.033.3779264
TCCGATC45450.033.0577743
GATCTGG10150.032.015186
TCTTTTT2850.031.8762688
CTTCCGA49200.030.4671061
CGATCTG23800.030.0964955
ATCTTAC1052.0550942E-829.9496827
ATAAGAA2250.027.9530374
ATAACTA755.6461744E-527.9530375
CGATCTA10550.027.8205575
GAGTGTA4250.027.23324870
ACCGGTA2200.027.00543415
CTAGCTA657.9437153E-426.92852840
TACTGGC658.031504E-426.8779210
TCTAGGC1056.863902E-726.6219393
Produced by FastQC (version 0.11.2)