FastQCFastQC Report
Fri 3 Aug 2018
H3VNWAFXY_n02_adr3wtzt14s3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VNWAFXY_n02_adr3wtzt14s3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1375826
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG46070.3348533898908728No Hit
TCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCACAC36170.262896616287234No Hit
CTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGC32610.23702125123380427No Hit
TCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTC31880.23171534772565716No Hit
CGTCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCAC27890.20271458745509968No Hit
CGTCTAGATCGGAAGAGCACACGTCTAGATCGGAAGAGCACACGTCTAGA15710.11418595083971374Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
ATCTAGACGTGTGCTCTTCCGATCTAGACGTGTGCTCTTCCGATCTAGAC14950.1086619965024647Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
ATCTAGGACTACCATGGTTGCAACGGGTAACGGGGAATCAGGGTTCGATT14910.10837126206366213No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCATTAT400.002442094534.9335371
GCCAATA605.040056E-429.1112792
TTTCGGA3300.028.5819851
CATACTA755.6491608E-527.95534711
TACAATA1004.4919398E-727.9516938
TGGTATA808.559719E-526.29974263
GATAATT808.749726E-526.21498518
ACGATAG4350.025.80155269
ATAGACA1101.0338954E-625.406213
ACCGGTA2800.024.9633915
TTCGGAA3800.024.8211982
ATATAAG2950.023.6837542
CTCTAGG901.9538743E-423.2890221
GGTAATA3050.022.92020818
GGTATAC1403.2274875E-722.54263564
AACTCCA3700.021.7192239
AATACGC3250.021.51535221
GACCGTA1304.4104127E-621.5013057
CATACAT3100.021.44447931
ATTTTAA1154.153735E-521.2814922
Produced by FastQC (version 0.11.2)