FastQCFastQC Report
Fri 3 Aug 2018
H3VNWAFXY_n02_adr3wtzt14s39.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VNWAFXY_n02_adr3wtzt14s39.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences158714
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG21621.3621986718247916No Hit
CTTCCGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN13000.8190833826883577No Hit
GTGCTCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN5040.31755232682687096No Hit
GGGGGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN3870.24383482238491877No Hit
GAAGAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN2520.15877616341343548No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCCGT200.006897046752.57972357
ATTTACG200.00692795752.52033246
AACTGAC200.00692795752.52033250
CCTACAG200.00696933152.4413535
CTAGGAC200.00696933152.4413533
TTTCGGA608.367351E-1152.441351
ATTGTGT404.5218974E-543.8329661
TAAAATG404.5623416E-543.7669451
TCATTCA404.5826757E-543.7340135
GGATTAT404.6030847E-543.701136
GATTATG404.6030847E-543.701137
TGGGTAA350.001251698440.0004340
TCGTTCT459.157446E-538.88930541
ATTATGG459.2185735E-538.8454488
TGGTGCT459.2185735E-538.84544812
TATGTAG459.2185735E-538.84544825
ATCTAAC556.7059045E-638.1391687
TGAATGT400.002385022435.07959469
GCCCTAC400.002393868335.0531556
CTTATTG501.687239E-435.05314658