FastQCFastQC Report
Fri 3 Aug 2018
H3VNWAFXY_n02_adr3wtzt14s37.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VNWAFXY_n02_adr3wtzt14s37.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences456334
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG20500.44923236050787363No Hit
TCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCACAC4590.10058422120639707No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCTAGG501.7282099E-434.93121
GTTTATA703.5318884E-529.9410291
GGCTGTA500.0071487728.04447461
ACCGTAT500.007190328.01122542
TTACACT500.007273931527.944964
AGGACTA1508.9858077E-1025.6162155
GACCATG700.001238229124.95413611
TAGGACT1551.3133103E-924.7898854
GTCCTAG1151.5192363E-624.2999631
AGAAACG1451.6905688E-824.1794667
TAGTATC1202.1999094E-623.2874662
TAGACCC952.8138698E-422.061814
GAATCAG1755.2241376E-922.00012435
TCTAGCG800.002692446321.834878
TAGATAC1454.6796958E-721.6814355
CTTGTGT4700.021.63289870
GGACTAC2108.54925E-1121.6240776
TCGGGTA1304.346908E-621.53007725
TCTAGGG1304.4068074E-621.4961222
CTAGGAC1656.911978E-821.1704253
Produced by FastQC (version 0.11.2)