FastQCFastQC Report
Fri 3 Aug 2018
H3VNWAFXY_n02_adr3wtzt14s35.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VNWAFXY_n02_adr3wtzt14s35.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences377547
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG18250.4833835257597068No Hit
ATCTAGGACTACCATGGTTGCAACGGGTAACGGGGAATCAGGGTTCGATT5710.15123944833358496No Hit
GTATAGTACTAACCACAATTGTAAGTTGTACTACCCGTATGAAGCACAAG4000.1059470741391138No Hit
CCTTAGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCT3780.10011998506146255No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAGCAG400.002399199235.05208662
ATTATCA400.002406709135.02976642
CTCAATA450.00427253531.1574167
ACTAGCC450.004316015631.09302326
CTAACAT450.004346322331.0486033
ACGTATT500.007262336627.9526220
CAACCCA500.00727360427.9437474
TAGGGGT500.00727360427.9437474
TAATGGA500.00727360427.9437474
TCTATGC906.2539257E-627.1675288
GTCTAGC657.907029E-426.94597443
TCTGAGG658.040346E-426.8689888
GTCCTTA658.040346E-426.8689881
CAATAAT851.297144E-424.73083149
ACCTGAA750.001851626623.2864551
TATACAT750.001851626623.2864555
ATCTATG1052.066435E-523.2864537
ACCTTTA1052.066435E-523.2864538
TACCGTG901.9509566E-423.2864537
ATACCTT1102.960389E-522.227986
Produced by FastQC (version 0.11.2)