FastQCFastQC Report
Fri 3 Aug 2018
H3VNWAFXY_n02_adr3wtzt14s33.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VNWAFXY_n02_adr3wtzt14s33.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences605903
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG68801.135495285549007No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCTG22100.032.0908625
TTCCGAT55650.031.951212
CCGATCT55300.031.8375854
TCCGATC57050.031.1671333
CTTCCGA58700.030.5290991
ATCTGGG4600.029.6199557
GATCTGG11250.029.5018126
GATCTAG4850.028.8134926
AGGGTCC500.007270923427.9490855
ACTCACG500.007270923427.9490858
ATCATAC500.00727443227.9463233
ATCTGGT2800.027.4499957
ATCTAGC2150.025.9991517
CTATACA959.525054E-625.7400364
ATATAAG959.525054E-625.7400362
CGATCTC15850.025.3481465
AGGTGTA6650.025.32483770
CGATCTA14950.024.5372415
TCTAGGC1001.4165837E-524.455458
TCTGGGG2051.8189894E-1223.8589768
Produced by FastQC (version 0.11.2)