FastQCFastQC Report
Fri 3 Aug 2018
H3VNWAFXY_n02_adr3wtzt14s31.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VNWAFXY_n02_adr3wtzt14s31.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences246698
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG23330.9456906825349212No Hit
TCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCACAC3200.12971325264088074No Hit
CTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGC3110.12606506741035597No Hit
TCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTC3030.12282223609433396No Hit
CGTCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCAC2690.10904020300124039No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGCTA150.002238508369.871918
TTGTACG350.001251185440.01416845
ATAGGGT350.001264711339.9268044
AAGGTAC459.275165E-538.8177227
TTTCGGA951.891749E-1036.774691
GCTTGTA400.002413518535.00388743
AGGTACG501.7240882E-434.9359558
TCTTTTT1500.034.9359558
TAACAGA400.002436637934.935956
TATCCGC400.002436637934.935956
CTTTTTT1600.032.7524579
ATCTTTT1750.031.941447
CTAAGCA553.0172418E-431.7599564
TTCGGAA1109.1677066E-1031.7599562
CAAAGTC450.0043133531.09189626
GTATTAG450.00433905631.0541781
CTAACGT450.00433905631.0541784
TATTCTA450.00433905631.0541785
ACGCAGT703.515018E-529.95236812
ACAAAGT604.988268E-429.14865125