FastQCFastQC Report
Fri 3 Aug 2018
H3VNWAFXY_n02_adr3wtzt14s27.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VNWAFXY_n02_adr3wtzt14s27.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences402778
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG12230.30364121178415904No Hit
ATCTAGGACTACCATGGTTGCAACGGGTAACGGGGAATCAGGGTTCGATT7600.1886895510678339No Hit
GTTATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACACACGA5980.14846888360337457No Hit
GTATAGTACTAACCACAATTGTAAGTTGTACTACCCGTATGAAGCACAAG5700.1415171633008754No Hit
GTCTTGTACCGACGACAGATCTTTCAAATGTCTGCCCTATCAACTTTTGA5000.12413786254462755No Hit
GTCCTAGATACTACCATCAAAAGTTGATAGGGCAGACATTTGAAAGATCT4800.11917234804284246No Hit
CCTTAGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCT4800.11917234804284246No Hit
GTCTTACGACGGTCCAAGAATTTCACCTCTCGCGTCGTAATACTAATGCC4610.11445510926614662No Hit
GTACAAGACCATACGATCTGCATGTTATCTAGAGTTCAACCAATATAACG4390.108993043314183No Hit
GTACTATTACTTTGAACAAATTAGAGTGCTTAAAGCAGGCTTCAAATGCC4340.10775166468873672No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACCTA150.002238206869.8840565
CTATAGG352.1066973E-549.9097861
TATAGGT350.001265698739.927833
ACGTATG400.002424387734.97828321
TAAGGGG957.734343E-933.1029745
AGGGTTT450.004329825831.07338115
TAGACCA605.029819E-429.1140424
ACGTGTA4100.029.0710270
GTACCGA2550.028.7757856
TCTGCGG755.6399007E-527.953628
TCTAGGC500.007261827.953628
CGTGTAG1900.027.67597470
TACGTGT4350.027.39620469
TTGTACC2600.026.87454
TGTACCG2900.025.3028475
GTACTAG700.001237722124.9548931
CCGATAT1151.4795005E-624.37246152
TTACGTG4900.024.31750768
CTTGTCT2900.024.0979489
TTGTCTC2800.023.71065910
Produced by FastQC (version 0.11.2)