FastQCFastQC Report
Fri 3 Aug 2018
H3VNWAFXY_n02_adr3wtzt14s25.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VNWAFXY_n02_adr3wtzt14s25.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6659
Sequences flagged as poor quality0
Sequence length76
%GC86

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG375256.34479651599339No Hit
GGGGGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN5818.725033788857186No Hit
GGGGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1462.192521399609551No Hit
CTTATACACATCTGACGCTGCCGACGAATAGAGAGGTGTAGATCTCGGTG801.2013815888271513No Hit
ATACACATCTGACGCTGCCGACGAATAGAGAGGTGTAGATCTCGGTGGTC290.4355008259498423No Hit
CTTATANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN70.10512088902237572No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGATC252.1908311E-456.00000438
GAGGTGT252.1908311E-456.00000433
ATCTCGG252.1908311E-456.00000442
GTCGCCG252.1908311E-456.00000451
AGAGGTG252.1908311E-456.00000432
GTATCAT252.1908311E-456.00000457
AGGTGTA252.1908311E-456.00000434
CCGTATC252.1908311E-456.00000455
CATTAAA252.1908311E-456.00000461
TCTCGGT252.1908311E-456.00000443
AATAGAG252.1908311E-456.00000427
ATTAAAA252.1908311E-456.00000462
CGTATCA252.1908311E-456.00000456
TATCATT252.1908311E-456.00000458
ATAGAGA252.1908311E-456.00000428
AGAGAGG252.1908311E-456.00000430
ATCATTA252.1908311E-456.00000459
TAGAGAG252.1908311E-456.00000429
GAGAGGT252.1908311E-456.00000431
GCCGTAT252.1908311E-456.00000454