FastQCFastQC Report
Fri 3 Aug 2018
H3VNWAFXY_n02_adr3wtzt14s22.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VNWAFXY_n02_adr3wtzt14s22.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences501031
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG21040.419934095894266No Hit
GTCTTACGACGGTCCAAGAATTTCACCTCTCGCGTCGTAATACTAATGCC6340.12653907642441287No Hit
TCGTAAGACTAACTTAAGCGAAAGCATTTGCCAAAGATGTTTTCATTAAT5450.10877570449732651No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCTTG150.002231781469.93764522
GACTAAT459.1751004E-538.90089444
AGTATAA450.004344502531.0536042
AGGTGTA4200.030.0525670
TTAATAC703.529869E-529.9445483
CTAACGC500.00716905428.02883753
GTATAAT658.031565E-426.8765341
CTTTAGA658.031565E-426.8765341
TATTGTA658.037189E-426.8733125
GGTTAGC808.632438E-526.2612647
TAGGGTG1207.519702E-826.2014795
ATAGGGT1101.0307995E-625.4074954
ATTCATT851.3149976E-424.68387420
TAGGTGT5150.024.50888469
GGGGTTA901.9219768E-423.34053645
CAGACGT901.9424023E-423.30416314
TCTAGGC901.947142E-423.2957848
AGTGAGG750.001849607623.2929946
TAAAGTG1253.134137E-622.3585975
GGTTAAT1102.9107387E-522.27960434
Produced by FastQC (version 0.11.2)