FastQCFastQC Report
Fri 3 Aug 2018
H3VNWAFXY_n02_adr3wtzt14s21.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VNWAFXY_n02_adr3wtzt14s21.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences757190
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCACAC26930.3556571005956233No Hit
TCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTC25140.3320170630885247No Hit
CTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGC23810.31445211901900444No Hit
CGTCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCAC22480.29688717494948424No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG14790.19532746074301033No Hit
ATCTAGGACTACCATGGTTGCAACGGGTAACGGGGAATCAGGGTTCGATT11740.15504694990689258No Hit
CGTCTAGATCGGAAGAGCACACGTCTAGATCGGAAGAGCACACGTCTAGA10200.1347085936158692Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
GTATAGTACTAACCACAATTGTAAGTTGTACTACCCGTATGAAGCACAAG10190.1345765263672262No Hit
ATCTAGACGTGTGCTCTTCCGATCTAGACGTGTGCTCTTCCGATCTAGAC9930.13114277790250795Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
GTTATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACACACGA9700.12810523118371875No Hit
GTCTTGTACCGACGACAGATCTTTCAAATGTCTGCCCTATCAACTTTTGA8480.11199302684927165No Hit
GTCCTAGATACTACCATCAAAAGTTGATAGGGCAGACATTTGAAAGATCT8360.11040821986555555No Hit
CCTTAGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCT8320.1098799508709835No Hit
GTACAAGACCATACGATCTGCATGTTATCTAGAGTTCAACCAATATAACG8300.10961581637369748No Hit
GTCTTACGACGGTCCAAGAATTTCACCTCTCGCGTCGTAATACTAATGCC7680.10142764695783094No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATACTA350.001265937339.93165611
CGAGTAA753.482637E-837.40006370
GGTCCAA2450.035.65326311
ACATAAT1253.6379788E-1233.66005769
GGGATTC1700.032.89010616
GACACAT751.4725101E-632.71724366
TGGATTA552.9718218E-431.8547750
AGTAACC450.00427450731.1592866
GTGTAAT450.004353315631.0431821
TCACCTC3000.030.29832523
AAGAATT2950.029.61503416
AGAATTT3000.029.12144917
TCATACT605.031889E-429.11682910
TTATACT605.0435826E-429.1052884
TCGCGTC2800.028.73962830
CAAGAAT3100.028.18204715
GACGGTC3350.028.1585228
GTAACCC500.007145358728.05004970
TTCCTAC755.525547E-528.0433563
TACTAGC500.007209448627.99879331
Produced by FastQC (version 0.11.2)