FastQCFastQC Report
Fri 3 Aug 2018
H3VNWAFXY_n02_adr3wtzt14s1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VNWAFXY_n02_adr3wtzt14s1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences214359
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG11640.5430142891131233No Hit
GTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACCTGCACGAAT2160.1007655381859404No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTTCA200.00697037952.44684613
TAGATAA305.919791E-446.580432
ATCTGTG757.6033757E-1041.934087
TAGGATG459.256108E-538.8278475
CTAGGAC654.899521E-737.633153
TAGTCCA553.0110776E-431.768247
GTGGATA450.00427320931.14914169
GAGGTAT450.004284922531.1317350
GGTTTCT450.004296662331.1143441
TGTACTG450.004332033531.0622795
TCTAGGA802.4889487E-630.5684052
TTCCGAT18750.030.3704382
CGATCTG8200.029.831155
CCGATCT18850.029.661594
TCCGATC19200.029.48493
CTTCCGA19500.029.3815021
TACCCAA605.013621E-429.1208885
ATCTAGC853.982952E-628.7782887
AGGTATC500.00716134528.0263951
TGCTCCC500.007220206327.97945818
Produced by FastQC (version 0.11.2)