FastQCFastQC Report
Fri 3 Aug 2018
H3VNWAFXY_n02_adr3wtzt14s19.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VNWAFXY_n02_adr3wtzt14s19.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences818627
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG27710.3384935996491687No Hit
TCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCACAC18010.2200025164085719No Hit
TCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTC17240.21059652320287503No Hit
CTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGC17070.20851987535226665No Hit
CGTCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCAC15040.18372225690088403No Hit
CGTCTAGATCGGAAGAGCACACGTCTAGATCGGAAGAGCACACGTCTAGA9670.11812461597284234Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
ATCTAGACGTGTGCTCTTCCGATCTAGACGTGTGCTCTTCCGATCTAGAC8290.10126712165613888Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACAATA652.1243326E-532.2595258
TATTACA450.00427682431.15618966
GTATATG802.499808E-630.5726131
ATAGTAC906.2533854E-627.1756533
ATATCGC700.001214690525.0399165
AGTCATG700.001226730924.99758328
GTCATAT700.001238339724.9572331
TTAGTCA750.001836433123.32422326
CTTGTCT1052.0627132E-523.2985469
GTATTAA750.001850607723.2934191
GTGTAGG1102.960081E-522.2346271
GGGTATT952.7996342E-422.08203316
GTACAAA1905.7480065E-1022.067451
TATGAAA800.002642589621.9083168
ACACCGT800.002689232121.8423886
TACAGGA1807.401468E-921.3522992
GTCCAAT850.00382697220.5530151
TAGACGA1055.4399465E-420.01277546
TAAAGGT1055.5268215E-419.9657884
CCAATAC1055.5268215E-419.9657883
Produced by FastQC (version 0.11.2)